TỈ LỆ VÀ ĐỊNH DANH CHỦNG NẤM CANDIDA GÂY VIÊM ÂM ĐẠO BẰNG MÔI TRƯỜNG BRILLIANCE CANDIDA AGAR VÀ KỸ THUẬT KHỐI PHỔ MADLI – TOF

Abstract

Objectives: Research on Candida vaginitis in women of childbearing age is essential for diagnostic, therapeutic and prophylactic activities. The identification of strains by culture method in Brilliance Candida Agar has not been reported in many studied in Vietnam. This study determined the prevalence of vaginitis caused by Candida and some clinical characteristics of patients visiting at the National Hospital of Dermatology and Venereology. To identify some Candida strains on Brilliance Candida Agar and MADLI-TOF mass spectrometry.

Materials and methods: A cross-sectional study of 392 female patients aged 18-49, diagnosed with vaginitis at the National Hospital of Dermatology and Venereology from August 2022 to June 2023. In 392 patients with positive results by using microscopic specimens, randomly took 50 samples of vaginal discharge cultured techniques by fungal cultured in Saboura colony growth medium, colonies were cultured fungal in Brilliance Candida Agar anonfirmed by MADLI – TOF mass spectrometry.

Results: The rate of vaginal candidiasis is the highest among causes vaginal discharge syndrome was 32.1%. In 50 case of Candida spp. infection identified on Brilliance Candida Agar and MADLI-TOF mass spectrometry, Candida albicans a were found to be 78%, Candida glabrata was 18%, Candida parapsilosis was 4%.

Conclusion: The rate of Candida vaginitis is highest among the causes of vaginal discharge syndrome, in which Candida albicans strains account for the highest rate. Isolation of strains through Brilliance Candida Agar medium showed very high accuracy when compared with MADLI – TOF mass spectrometry.